You should be able to determine which by looking at the \(R_f\) value. If the compound likes the stationary phase, it will stick to it, which will cause it to not move very far on the chromatogram. How well the compound likes the stationary phase.If the compound is soluble in the solvent, it will travel further up the TLC plate.
How fast the compounds travel up the plate depends on two things: If you know that one component of a mixture is insoluble in a given solvent, but another component is freely soluble in it, it often gives good separations. If the value is 1, you need to decrease your solvent polarity because the compound was not able to separate. Bea also calculates the volume of the sugar cone and finds that the difference is < 15, and decides to purchase a sugar cone. If the value is 0, you need to increase your solvent polarity because the sample is not moving and sticking to the stationary phase. The volume of the waffle cone with a circular base with radius 1.5 in and height 5 in can be computed using the equation below: volume 1/3 × × 1.5 2 × 5 11.781 in 3. When performing your experiment, you do not want your values to be 0 or 1 because your components that you are separating have different polarities. \(R_f\) valu es range from 0 to 1 with 0 indicating that the solvent polarity is very low and 1 indicating that the solvent polarity is very high. Varying the ratio can have a pronounced effect of \(R_f\). A common starting solvent is 1:1 hexane:ethyl acetate. As with plate selection, keep in mind the chemical properties of the analytes. In Imperial or US customary measurement system, the density is equal to 0.117326 pound per cubic foot lb/ft³. density of propane, gas is equal to 1.87939 kg/m³ at 0☌ (32☏ or 273.15K) at standard atmospheric pressure. Proper solvent selection is perhaps the most important aspect of TLC, and determining the best solvent may require a degree of trial and error. Propane, gas weighs 0.00187939 gram per cubic centimeter or 1.87939 kilogram per cubic meter, i.e. Nucleic acids, nucleotides, nucelosides, purines, pyrimidinesĬhromatographic Columns is a good reference to learn more about the different types of columns and stationary phases. Steroids, amino acids, alcohols, hydrocarbons, lipids, aflaxtoxin, bile, acids, vitamins, alkaloidsįatty acids, vitamins, steroids, hormones, carotenoidsĬarbohydrates, sugars, alcohols, amino acids, carboxylic acids, fatty acidsĪmines, alcohols, steroids, lipids, aflatoxins, bile acids, vitamins, alkaloids \): Stationary phase and mode of separation Stationary Phase